We combined our unique CIM monolith technology with a standard multi-well plate design. Whether your goal is to screen multiple chromatographic conditions at once or high throughput purification of your target molecule, our CIM plates offer an answer in either a 24-well or 96-well plate design. A wide range of monolith volumes and available CIM chemistries allow for the best fit for your large biomolecule such as viruses, nucleic acids, exosomes, bacteriophages, etc.
Each well in our monolithic plates is prefilled with a defined amount of monolith to enable uniform high flow rates across the plate. The properties of the monoliths in the wells are comparable to our preparative chromatographic monolithic columns. This makes plates an ideal tool for screening different parts of your chromatographic process.
CIM plates can be disposable or multi-use and are made from medical grade polypropylene (PP), a material that prevents target molecules from binding to the plastic. They are manufactured according to ANSI standards and are automation compatible. Nevertheless, they can also be operated manually with a vacuum, centrifuge or positive pressure.
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FAQ About CIM® Monolithic Well Plates
The CIM® Monolithic Well Plates are recommended for screening samples and process conditions, purification process optimization and high-throughput purification of small volume of samples. They are suitable for a wide range of applications, including:
- Parallel screening and optimization of chromatographic conditions in downstream process development (e.g., conductivity, pH)
- Scale-down experimental work
- Determination of dynamic binding capacity (DBC)
- Determination of optimal residence time
- Optimization of the capture and polishing step
Yes. The plates feature the same CIM monolith technology and chemistries as our preparative CIMmultus columns. Matching chromatographic characteristics of monoliths enables you a straightforward transfer from one scale to another.
We suggest these chemistries as a starting point:
Adeno-associated Virus:
- AAV capture step purification using cation exchange chromatography: CIM SO3 Monolithic Well Plates
- AAV polishing step purification using anion exchange chromatography: CIM QA HR Monolithic Well Plates or mixed-mode chromatography: CIM Prima T Monolithic Well Plates
Plasmid DNA:
- pDNA capture using weak anion exchange: CIM DEAE Monolithic Well Plates
- pDNA polishing using hydrophobic interaction chromatography: CIM C4 HLD Monolithic Well Plates
Messenger RNA:
- mRNA capture step purification using affinity chromatography for mRNA with poly-A tail: CIM Oligo dT Monolithic Well Plates or mixed-mode chromatography for any RNA modality: CIM Swiper Monolithic Well Plates
- mRNA polishing step purification using reverse phase chromatography: CIM SDVB Monolithic Well Plate
Lipid Nanoparticles:
- LNP purification using hydrophilic chromatography: CIM OH Monolithic Well Plates (6 μm channels)
Lentivirus:
- Lentivirus capture step purification using strong anion exchanger: CIM QA Monolithic Well Plates
Bacteriophages:
- Bacteriophage capture and polishing using hydrophilic chromatography: CIM OH Monolithic Well Plates, and anion exchange: CIM QA Monolithic Well Plates or mixed-mode chromatography: CIM PrimaS Monolithic Well Plates
Extracellular Vesicles | Exosomes:
- Strong anion exchanger: CIM QA HR Monolithic Well Plates
Plates can be operated standalone with:
- Vacuum manifold to suck solution through the monolith. Any plate manifold that has a gasket is compatible with CIM® Monolithic Well Plates;
- Positive pressure manifold to push solution through the monolith;
- Centrifuge. It should be able to accept deep well plates in appropriate buckets.
Follow this detailed step-by-step video guide on how to operate CIM® Screening Plates with different equipment.
Yes, the plates are made according to ANSI standard and are compatible with liquid handlers.
Yes, all our well plates are based on the monolithic technology, which is reusable.
Depending on the plate chemistry, it can be cleaned with various concentrations of NaOH solutions. You can find more info on cleaning the Instructions for Use (IFU) of your well plate.
Re-usability of the plate will depend on the quality of the sample loaded on it. Usually the “dirtier” the sample the shorter the life span. Plate maintenance also plays an important role. If the plate is well cleaned after each use, the performance and lifespan are preserved significantly. We recommend following the Instructions for Use for your specific plate and optimize the cleaning for your particular sample.
No. Single chemistry plates are a standard product. Making a custom plate with two chemistries would be costlier than buying two single chemistry plates.
We suggest trying CIM® Octa miniaturized columns, as they offer a flexible and efficient approach to chromatography. You can arrange up to 12 strips, each containing 8 columns, on a 96-array plate.
