Sartorius BIA Separations
DEAE (diethylamine)
- Weak anion exchanger
- Working range, pH 2–9
- Binds molecules with predominantly negative charge, repels molecules a predominantly positive charge
- Use for capture, intermediate, or polishing steps in either bind-elute or flow-through mode
- High capacity for plasmid DNA
- Sanitizable with 1 M NaOH
Applications:
- Screening of chromatographic conditions (example binding, washing, elution conditions)
- High throughput of pDNA purification