PostersAn industrial purification platform for AAV
polishing is performed with a CIMmultus SO3 cation exchange monolith. The AAV fraction from the ... effective for all AAV serotype tested to date. Cell lysate is directly subjected to column ...
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PostersInfluence of chromatographic parameters on empty/full AAV separation
Monoliths are commonly used for separation of empty and full AAV capsids on analytical and ... preparative scale. PrimaS approach for AAV separation employs an ascending pH gradient, which can be ...
PostersSeparation of empty and full AAV capsids by multimodal metal affinity chromatography
Removal of empty capsids is a particular goal of AAV purification. Multimodal PrimaT ligand offers ... clearance of contaminating DNA. PrimaT works for different AAV serotypes - for example AAV 2/8 and AAV ...
PostersImplementing Chromatographic Methods for Evaluation of Large Scale Monolithic Columns for AAV Capsids Separation
therapeutically relevant adeno-associated virus (AAV) vectors, used in gene therapy. Beside process- and ... scalability of AAV downstream processing. This study presents the evaluation of highly reproducible (HR ...
PostersChromatographic separation of full and empty AAV8 capsids
process. This poster demonstrates how CIMmultus™ QA monolithic columns can be used to remove empty AAV ... Adeno-associated virus (AAV) vectors of various serotypes are considered to have high potential for ...
PostersHEK293-derived Adeno Associated Virus (AAV) purification: comparison of small scale laboratory production towards industrial format using monoliths
rAAV virus purification platform, which can be used for several AAV serotypes with various inserts ... . Lysed harvest and supernatant of rAAV9 were first captured and concentrated on CIMmultus™ OH column ...
PostersCIM PrimaT - New multimodal ligand for separation of empty and full AAV9 capsids
Kryptonase treatment. Capture step was performed on CIMmultus SO3 cation exchange column. AAV elution ... -chelating ability. This was sucessfully exploited for AAV capsid separation. ...
PostersMonolithic Columns as Downstream Processing Solutions for Lentiviral Purification
capabilities compared to more commonly used AAV. As a result, several therapies using lentiviral vectors are ... promising initial results. The chemistries have been successfully upscaled to CIMmultus monoliths and the ...