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Monolithic Columns as Downstream Processing Solutions for Lentiviral Purification

Lentiviral vectors are efficient tools for transfer and stable integration of large gene inserts into the genomes of both dividing and non-dividing cells. Third generation of lentiviral vectors, which uses a fraction set of HIV genes, is replication incompetent and self-inactivating, offering a relatively safe tool for academic and industrial use, while offering a larger gene transfer capabilities compared to more commonly used AAV. As a result, several therapies using lentiviral vectors are already approved or in clinical trials, with primarily ex-vivo use. Limitations for more widespread use, as well as development of in-vivo therapies, however remain, with one of the causes being downstream purification of lentiviruses. Here we showcase a process development for CIM monolithic columns for use in downstream purification of lentiviruses. Several CIM monolithic chemistries have been tested to determine their performance for lentiviral purification, with three chemistries showing potential for further use. Of the three chemistries, two have been selected for further development. Several modifications of the two chemistries have been prepared and tested on CIMmic columns with promising initial results. The chemistries have been successfully upscaled to CIMmultus monoliths and the results from initial findings confirmed. Additional experiments are required to confirm the findings and perform optimization. The full downstream process will be developed once the most suitable chemistry is selected. During preparative, multi-angle light scatter (MALS) was used as indicator of viral presence, while analytics, used in the process development, were ddPCR and infectivity test.