Separation of empty and full AAV capsids by multimodal metal affinity chromatography
AAV2 8 or AAV 2 9 clarified harvest from Sf 9 cells was first processed by tangential flow filtration TFF coupled with Kryptonase treatment to reduce host cell DNA. Initial AAV capture step was performed on CIMmultus SO3 cation exchange column. After elution with sodium chloride gradient AAV fraction was cleared of DNA and protein contaminants. Separation of empty and full AAV capsids was performed by multimodal metal affinity chromatography with CIMac and CIMmultus PrimaT.