CIMmultus PrimaS purifies large single-stranded mRNA (ssRNA) under aqueous conditions at ambient temperature. It removes dsRNA, DNA, proteins, and endotoxins while fractionating ssRNA in order of increasing size. It can be used for one-step purification of research grade ssRNA or as a high-resolution capture step in a multi-step purification process. It also enables fast high-resolution analytical characterization of in vitro transcription mixtures, partially purified samples, chromatography fractions, and formulated drug substance.
CIMmultus C4 HLD is a hydrophobic interaction chromatography monolith for purification of nucleic acids. Sample is applied at high ionic strength in salts that precipitate RNA. The column is eluted with a decreasing salt gradient. The majority of DNA, dsRNA, and short transcripts elute earlier than intact ssRNA. The majority of proteins and aggregates bind very strongly and are eliminated by a cleaning step with NaOH. CIMmultus C4 HLD can be used to produce research grade ssRNA from in vitro transcription (IVT) mixtures but gives its best results as a polishing method.
CIMmultus Oligo dT is a hybridization-affinity chromatography monolith for purification of mRNA that terminates in a poly-A tail. Non-RNA contaminants flow through the column during sample loading. RNA with a poly-A terminus binds. The column is washed then the mRNA is recovered in a single elution step at neutral pH. CIMmultus Oligo dT can be used for one-step purification of research grade ssRNA, as a high-resolution capture method in a multi-step purification process, as a polishing method, or as an analytical method for estimating quantity and purity of mRNA in a sample. It can be used with mRNA up to 10 kb or more.