Application Notes
2008
Diluted samples of live attenuated measles and mumps virus were each loaded on CIM® DEAE Disk. Concentrated eluates of viral RNA were subjected to molecular detection by PCR. It was demonstrated that enrichment of viral RNA on a CIM® DEAE Disk prior PCR is feasible and successful.
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A Hemoglobin A1c reference standard was loaded on CIM® SO3 monolithic column and eluted in a mixed stepwise and linear gradient. HbA1a, HbA1b and HbA0 variants were separated and a complete determination of HbA1c (including equilibration) was obtained within 1.1 minute.
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A mixture of IgG, HSA and IgM standard was loaded on CIM® EDA Disk and eluted in linear salt gradient at a flow rate of 4 mL/min (12 CV/min). A complete separation of IgM from IgG and HSA was obtained within 1.5 minute.
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A supernatant from Phanerochaete chrysosporium cultivation was loaded on CIM® QA Disk, and elution was effected by a linear gradient at a flow rate of 3 mL/min (9 CV/min). Baseline separation of isoenzymes H2, H6/H7, H8 and H10 was achieved in less than 3 minutes.
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A mixture of 8mer, 10mer, 12mer, 14mer, 15mer and 16mer Oligodeoxynucleotides was loaded on CIM® DEAE Disk and eluted in linear gradient mode at a flow rate of 6 mL/min (17 CV/min). Separation of all nucleotides could be accomplished within 60 seconds.
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Immunoaffinity columns were prepared by immobilization of Protein G on CIM® Epoxy Disk, CIM® Epoxy tube (1 mL) and an activated, particle based agarose support. A comparison of productivity was performed by loading centrifuged human plasma and resulted in superior productivity of CIM® monolithic supports.