Downstream processing of viruses in virus vaccine or virus vector production accounts for up to 70% of the overall production costs. Immunoaffinity chromatography is a powerful purification technique due to its high specificity but is disadvantageous by the fact that the elution conditions are often detrimental for both the immobilized proteins and target antigens, especially viruses.
This application note describes the mumps virus purification using monolith-based immunoaffinity stationary phase and recently invented native elution of the bound viruses using amino acid solutions under physiological pH.
Human coronavirus OC43 (HCoV-OC43) is a frequent cause of respiratory tract illness, ranging from common cold to severe disease. The research on coronaviruses and medical application of coronaviral vectors/vaccines requires a quality material of high purity. Unfortunately, virus preparations are highly contaminated with cell debris and purification requires laborious, cost-ineffective procedures.
Here, we report a simple and efficient method for coronavirus concentration and purification by the example of HCoV-OC43. To achieve this, virus chromatography was performed on CIM QA monolithic columns (BIA Separations), with immobilized positively charged quaternary amines. The quality of the obtained virus stock was assessed with SDS Page electrophoresis, followed by Western blot analysis. Finally, infectivity of recovered virus was evaluated by titration.
Influenza vaccines are still predominantly produced in embryonated chicken eggs and the purification processes barely have changed during the years. There is a growing need for fast, efficient and economical vaccine production.
So far, monolithic supports have been used successfully in virus purification and concentration, as well as in the purification of virus-like particles (VLP) propagated in cell cultures.
Therefore, our aim was to prove the applicability of monoliths in purification of influenza virus A propagated in embryonated chicken eggs.
Adeno-Associated Virus (AAV)-based vectors of various serotypes are considered to have high potential in human gene therapy and genetic vaccination applications. During manufacturing of AAV vectors undesired, incomplete particles are co-produced. They lack recombinant viral genomes and consist of empty capsid proteins only. Empty capsids increase the required dose of AAV virus for medical applications and are thought to cause immunological reactions against the vector capsid, leading to unwanted side effects. Removal of empty capsids during manufacturing, as well as the ability to quantify the amount of empty AAV particle content in a formulation is hence a critical requirement for any AAV production process.
Current methods for preparative separation of empty capsids (CsCl or iodixanol gradients) are challenging to scale-up and are not suitable for large-scale production. Furthermore, analytical methods for detection of empty capsids and determination of full to empty particle ratio (electron microscope (EM) assay, total particle assay [ELISA] combined with genome copy titration [qPCR]) are time- and labour consuming, influenced by operator technique or do not provide readily available reagents for different serotypes of AAV.
A new approach for separation of full and empty AAV8 particles by exploiting minor charge differences is presented in this application note. By using linear gradient elution on a CIM QA Disk Monolithic Column, a simple, rapid and reproducible assay for analysis of AAV particles is introduced. The method was successfully applied to AAV8 particles prepared by two different manufacturing processes.
Determining the concentration of viruses is a crucial step in any production process. The most commonly used methods for virus quantification are either based on the infectivity of the virus (plaque assay, TCID50) determination of their genomic material (qPCR), or protein content (SRID, ELISA) and are very cumbersome and time consuming. HPLC analytical methods represent a fast alternative to these assays since they provide information on the virus content and purity in a matter of minutes. In addition to that, the data obtained is very reproducible and accurate.
For any kind of quantification, a calibration curve obtained with a virus standard is needed. The work presented in this application note shows the excellent performance of the CIMac™ Adeno Analytical Column – a monolith based anion exchange column, designed for fast and reproducible analyses of adenoviruses.
Due to increasing demands for the quantity of influenza vaccines and threat of pandemics novel approaches using cell based production of the virus and different purification methods started to emerge. In this application note, we present a chromatographic influenza virus vaccine purification method enabling the purification of different influenza A and B subtypes.
Recombinant baculovirus is extensively used for expression of recombinant proteins in insect cells. The appeal of baculovirus systems lies in their high level expression within an eukaryotic system, providing target proteins with appropriate posttranslational modifications. Recent approaches as vector in human gene therapy applications indicate a new dedication for baculovirus.
In any field of operation the increasing demand of highly pure baculovirus requests efficient, robust and scaleable purification strategies. Traditional techniques such as ultracentrifugation and tangential flow filtration are efficient in terms of virus concentration, but suffer from low yield and clearly lack robustness and scalability. In this application sheet we introduce a CIM monolith based purification process for infective baculovirus.
The protocol provides high recovery of active virus, efficient removal of host cell impurities, ease of use and straight forward scale up.
Orthoreoviruses are dsRNA, non-enveloped viruses that can cause severe enteric and respiratory infections in humans and other animals. It is speculated that these viruses might be an important zoonotic pathogen. As such, orthoreoviruses can cause infections of undetermined etiology which are difficult to resolve. Next-generation sequencing (NGS) is a new technology which enables gathering a huge amount of genomic information from a sample in a short period of time. NGS is being increasingly applied in animal screenings for pathogen discovery and has a great potential in clinical microbiological diagnostics. However, the preparation of high-quality and high-quantity nucleic acid samples is a major concern for efficient application of the method.
CIM QA® disk in combination with NGS was used for discovering a novel reovirus in stool samples of a child with gastroenteritis infection of undetermined etiology. Two different starting samples were compared: clarified stool suspension and supernatant from cell culture inoculated with clarified stool suspension.
Diluted samples of live attenuated measles and mumps virus were each loaded on CIM® DEAE Disk. Concentrated eluates of viral RNA were subjected to molecular detection by PCR. It was demonstrated that enrichment of viral RNA on a CIM® DEAE Disk prior PCR is feasible and successful.
Adenovirus vectors have proven as useful tool for gene therapy, vaccine therapy and basic biology studies. The increasing importance of the recombinant adenoviruses pushes the limits of research in the field of adenovirus purification methods. There is a global focus on large scale production of adenovirus vectors, providing high titres combined with fast, effective and reliable purification methods.
Because of the physico-chemical properties adenovirus vectors possess, they can effectively be purified using ion-exchange chromatography. Here we present a simple and rapid method for adenovirus vectors purification using ion-exchange CIM ®QA chromatographic supports (Figure 1). CIM® monolithic supports are a new generation of chromatographic supports able to meet the GMP and GLP requirements in the field of virus purification.
Adenoviruses are among the most commonly used vectors for the delivery of genetic material into human cells and as such there is demand for high-titre manufacturing processes. The key to the successful development of such processes are analytical methods that can be applied to the final purified samples and throughout the production process. Many conventional methods for quantitative analysis of adenoviruses are labour and time-intensive. For example, a plaque assay can take up to 7 days to perform, is prone to error and will only report the number of infectious and not total viral particles. The resolving power of the high-performance liquid chromatography (HPLC), on the other hand, permits separation of intact virus particles from other cellular contaminants or virus particle fragments.
Anion-exchange chromatography has already been applied to analyse various adenovirus preparations. The results from the anion-exchange HPLC methods can be obtained much faster, within minutes, thus allowing for a faster evaluation of different process steps. A method was designed and developed to quantify adenoviral particles using a strong anion-exchange CIMac™ Analytical column. Regeneration conditions were incorporated to extend the functional life of the column.
Virus like particles (VLPs) are particles that structurally resemble viruses, but do not contain any genetic material. They are formed when structural viral proteins spontaneously self-assemble in transfected cells. Extracts from expressing cells contain not only VLPs, but also cellular DNA and proteins. These need to be removed in order to obtain pure VLPs, which are then applied for the production of vaccines, as delivery systems, as well as in other fields of nanotechnology applications (for the application on DSP of Ad3 VLPs check the Application Note A029). The purity of the final VLPs product is evaluated by methods like SDS-PAGE, agarose electrophoresis, PicoGreen analysis, BCA or Bradford assay.
In this work, CIMac™ QA Analytical Column was used for in-process control of the adenovirus serotype 3 dodecahedric virus-like particles (Ad3 VLPs). Samples obtained from different purification steps were injected on the CIMac™ QA Analytical Column and elution profiles were compared.
Environmental water is contaminated with human enteric viruses through the discharge of sewage contaminated water. As a consequence, they are present in various environmental water sources: irrigation water, wastewater, recreational water, ground or subsurface water, and even drinking water. The continuous low level transmission of these viruses can result in the spread of some viral infections. The nature of most enteric virus diseases is such that they elude epidemiological studies. Improved detection of viruses that are present in low concentration could prevent a considerable number of infections. Among the most important human food-borne viruses are Noroviruses (NoVs), members of Caliciviridae family and hepatitis A virus (HAV) which can be the source of serious outbreaks.
CIM® monolithic columns in combination with ultracentrifugation and RT-qPCR were used for the concentration and detection of hepatitis A virus and feline caliciviruse, a norovirus surrogate. At the same time efficiency of newly developed method was compared with reference method, based on membrane filter.
One of the most important plant viruses causing great economical losses in potato production is the filamentous Potato virus Y (PVY); virion size is 740 nm × 11 nm. Preparation of the pure virus suspension is essential for in vitro characterisation of the virus and also in many applications (e.g. antibody production). Virus purification usually consists of complicated and time-consuming protocols involving several ultracentrifugation steps, which are needed for isolation of the virus from the complex plant tissue matrix.
Different column chemistries, mobile phases and sample preparation strategies were examined during the method development study. Based on the obtained results, an optimised purification method for PVY from plant tissue on a CIM® QA Disk Monolithic Column was designed. The presence of the virus in the chromatographic fractions was monitored with viral RNA quantitation (RT-qPCR), viral protein detection (SDS-PAGE) and observation of the viral particle integrity (transmission electron microscopy).