applicable plasmid DNA (pDNA). To guarantee the supply for the clinical trials and finally for the market new ... pDNA production processes, which meet all regulatory requirements, have to be developed ...
Preparative scale chromatographic separation of open-circular (oc) from supercoiled (sc) plasmid ... DNA (pDNA) isoforms has been already established on CIM® C4 with high ligand density (C4 HLD ...
conditions to separate plasmid DNA (pDNA) isoforms under overloading conditions, where supercoiled ... is beneficial for use of plasmids as vaccines, transfecting agents for production of gene therapy ...
(sc) plasmid DHA (pDLIA), using a monolithic column based on anion-exchange chromatography. This ... analytical method with UV detection allows distinguishing the plasmid isoforms by a NaCl stepwise ...
therapy purposes and genetic vaccination. In this case, plasmid DNA (pDNA) of high purity is required ... Plasmids are excellent genetic vectors and have been widely used in gene manipulation and ...
plasmid DNA (pDNA) production in supercoiled (sc) isoform, linearization and purification, 2) in ... toolbox and mRNA IVT synthesis for integrated mRNA production from pDNA to mRNA purification, including in ...