Since plasmid DNA (pDNA) as a pharmaceutical product has stringent requirements of purity and ... ligand density butyl-modified (C4 HLD) monolithic support is currently used in a polishing step of a pDNA ...
Application of plasmid DNA for gene therapy and vaccination has gained huge interest in last two ... decades. Topological homogeneity and impurity content are crucial for therapeutic usage of pDNA. Major ...
(pDNA) quality, with ability to separate pDNA isoforms (sc, oc, lin, multimer). Plasmid linearization ... Agarose gel electrophoresis (AGE) analysis is an important method for monitoring of plasmid DNA ...
PATfix analytical platform presented here uses CIMac pDNA column, to separate and characterize plasmid ... Optimizing processing steps in sc pDNA isolation is critical for obtaining good process yields as ...
Most commonly plasmids are manufactured by fermentation of E. coli. In the cells several isoforms ... of the plasmid are generated: supercoiled (sc), open circular (oc) and linear as well as dimeric ...
New development in the modern biotechnology increased the need for plasmid DNA (pDNA) with sizes ... above 10 kbp (large pDNA), but their chromatographic purification is often challenging due to low ...
One of the major requirements for pharmaceutical-grade pDNA is its high homogeneity, being mostly ... in supercoiled (sc) isoform. Chromatographic separation of sc pDNA from open coiled (oc) or linear ...
Linearised pDNA is currently the starting point of In-Vitro-Transcription processes to synthesize ... mRNA. Large scale purification protocols for manufacturing of pDNA used for Gene Therapy applications ...
conventional particle-based matrix as a plasmid DNA (pDNA) purification platform. By harnessing the ... differential affinity of pDNA, RNA. Host cell proteins and endotoxin to copper ions in the solution a majority ...
Application of plasmid DNA for gene therapy and vaccination has gained substantial interest in the ... pDNA. Downstream processing has major influence on achieving regulatory demands in pDNA production ...