PATfix analytical platform presented here uses CIMac pDNA column, to separate and characterize plasmid ... Optimizing processing steps in sc pDNA isolation is critical for obtaining good process yields as ...
Most commonly plasmids are manufactured by fermentation of E. coli. In the cells several isoforms ... of the plasmid are generated: supercoiled (sc), open circular (oc) and linear as well as dimeric ...
/s00232-013-9580-5
The use of plasmid DNA (pDNA) as a pharmaceutical tool has increased since it ... represents a safer vector for gene transfer compared to viral vectors. Different pDNA extraction ...
Černigoj
Electrophoresis. 2023; 1– 13
Increased need for plasmid DNA (pDNA) with sizes above 10 ... was to evaluate the mass balance and pDNA isoform composition at column outlet for plasmids of ...
New development in the modern biotechnology increased the need for plasmid DNA (pDNA) with sizes ... above 10 kbp (large pDNA), but their chromatographic purification is often challenging due to low ...
One of the major requirements for pharmaceutical-grade pDNA is its high homogeneity, being mostly ... in supercoiled (sc) isoform. Chromatographic separation of sc pDNA from open coiled (oc) or linear ...
(2010) 2039–2045Plasmid DNA (pDNA) used in vaccination and gene therapy has to be highly pure and ... purification of large molecules and nanoparticles seem to be a matrix of choice for pDNA purification. In ...
HPLC based analytical assay is a powerful technique that can be used to efficiently monitor plasmid DNA ... (pDNA) purity and quantity throughout the entire purification process. Anion exchange monolithic ...
Linearised pDNA is currently the starting point of In-Vitro-Transcription processes to synthesize ... mRNA. Large scale purification protocols for manufacturing of pDNA used for Gene Therapy applications ...
HiP2 Plasmid Process Pack™ produces low endotoxin, highly homogeneous supercoiled plasmid DNA (pDNA ... manufacturing of pDNA as raw material or drug substance. The advantage of monolithic columns allows ...