Technical Notes

CIMmultus® QA HR column is anion exchange chromatography monolithic column. One of its main applications is enrichment of AAV full particles. Empty and full AAV capsids have small difference in pI, which can be used for separation using its charge difference for elution. After samples are bound to the column at high pH and low conductivity, they are eluted with increasing linear salt gradient. Empty and full capsids elute at different conductivities due to their charge differences.

CIMmultus® QA HR can be used for polishing step of AAV samples. Polishing step is used as a second AAV chromatography step after capture step. In capture step we remove majority of impurities, which could co-elute with full AAV capsids in polishing step and reduce its purity.