Correctly Assess Critical Quality Attributes for AAV Upstream
Refining a robust AAV upstream strategy is a complex process. It involves optimizing multiple parameters, including cell line selection, media formulation, transfection, and plasmid ratios. To ensure success, you need to constantly monitor your progress by analysing crude harvest samples for productivity and empty-full content.
However, many analytical techniques demand specific sample purity and concentration for accurate results. For efficient testing of multiple samples, what’s needed is a high-throughput method that is also compatible with automation.
How to Get Accurate Data on Your Crude Harvest
Monolithic multi-well plates are an ideal solution due to their ability to streamline sample handling and facilitate efficient processing. Our method, developed using the CIM® SO3 0.2 mL Monolithic 96-well Plate (2 μm channels), enables high-throughput preparation of AAV samples for Mass Photometer analytics, ensuring precise and efficient assessment.
Learn more about optimizing your AAV upstream process with well plates by watching Refeyn‘s webinar, “Enabling Early AAV Characterization – The Role of Mass Photometry in Upstream Process Development,” and gain valuable insights to enhance your production strategy.
With the CIM® SO3 Monolithic Multi-Well Plate, you can get precise and efficient assessment of your AAV samples, saving valuable time and resources.
Key features include:
- High-Throughput: Process multiple samples simultaneously, accelerating your upstream process development.
- Simplicity of operation: No shaking nor incubation required.
- Automation Compatibility: Designed for use with vacuum manifolds, centrifuges, or robotic liquid handlers, it seamlessly integrates into your automated workflow.
- SO3 Chemistry: This strong cation exchanger is ideal for capturing and purifying AAV from complex crude lysates.
- Scalability: Results from the well plate can be easily transferred to larger-scale CIMmultus® columns, ensuring consistency from development to manufacturing.
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