The PrimaS HR line is a new generation of pre-packed chromatographic monolithic products developed to meet the most demanding separation and purification needs. HR stands for High Reproducibility between batches and different scales.

  • Multimodal chromatography ligand that combines elements of hydrogen bonding with weak anion exchange chromatography.
  • Selected to meet the need for highly reproducible purification processes (e.g., separation of full AAV capsids from empty AAV capsids)
  • Column release criteria based on the AAV8 elution profile.
  • AAV Binding at neutral or closer to neutral pH compared to QA-based columns: Multimodal properties of PrimaS HR allow binding of AAV in neutral or closer to neutral pH values compared to QA-based columns.
  • Reusability across multiple cycles reduces cost and supports high-throughput needs.
  • Endotoxin Removal: Provides superior reduction of endotoxin compared to strong anion-exchangers (e.g. QA).
  • cGMP-compliant: suitable for clinical and commercial production.

Products

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ImageProduct nameCatalog no.VolumeChannel sizeIFUInquiry
CIMmultus PrimaS® HR - 1 mL (2 μm)BIA-311.5119-21 mL2 μm
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CIMmultus PrimaS® HR - 4 mL (2 μm)BIA-414.5119-24 mL2 μm
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CIMmultus PrimaS® HR – 8 mL (2 μm)BIA-411.5119-28 mL2 μm
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CIMmultus PrimaS® HR – 40 mL (2 μm) – cGMPBIA-914.5119-240 mL2 μm
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CIMmultus PrimaS® HR – 80 mL (2 μm) – cGMPBIA-911.5119-280 mL2 μm
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CIMmultus PrimaS® HR – 400 mL (2 μm) – cGMPBIA-924.5119-2400 mL2 μm
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CIMmultus PrimaS® HR – 800 mL (2 μm) – cGMPBIA-921.5119-2800 mL2 μm
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CIMmultus PrimaS® HR – 4000 mL (2 μm) – cGMPBIA-934.5119-24000 mL2 μm
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CIMmultus PrimaS® HR – 8000 mL (2 μm) – cGMPBIA-931.5119-28000 mL2 μm
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FAQ About Custom Chromatography Services

What are the differences and similarities PrimaS and PrimaS HR?

CIMmultus PrimaS HR and CIMmultus PrimaS are both weak anion exchangers with hydrogen bond interactions, sharing the same chemical backbone. The differences between them arise from different release testing and criteria, leading to different reproducibility performance.

CIMmultus PrimaS is designed for purification of a wide range of biomolecules (such as mRNA, bacteriophage, AAV, etc.) and is suitable for robust separations and linear gradient methods, which allow for some variability in the elution of the target molecule. On the other hand, CIMmultus PrimaS HR product line is recommended for sensitive separations where reproducible chromatography is essential (e.g., AAV empty – full separation), as it ensures consistent column performance, regardless of batch or product scale.

What is the difference between CIMmultus QA HR and CIMmultus PrimaS HR?

The main difference is the ligand. QA HR is a strong anion exchanger, while PrimaS HR is a weak anion exchanger with hydrogen bonding interactions.

Which column is best suited for AAV E/F separation?

For AAV E/F separation, 3 different columns are available: CIMmultus QA HR, CIMmultus PrimaT, and CIMmultus PrimaS HR.

Based on experience, each AAV sample behaves differently, and the optimal polishing column may vary for different samples. Therefore, conducting a screening of all three columns is advisable, as each column possesses unique characteristics. A comparative evaluation of three CIMmultus polishing columns for enhanced vector enrichment and final product purity is demonstrated in this poster.

Is Specimen unit provided?

Specimen unit is provided only with cGMP CIMmultus PrimaS HR columns.