Monoliths as Stationary Phases for Separating Biopolymers – Fourth-Generation Chromatography Sorbents

G. Iberer, R. Hahn, A. Jungbauer

LC-GC Europe, February 2000

Monoliths are chromatography sorbents cast as a homogenous phase into chromatography columns as a single, continuous piece. In contrast, regular chromatographic sorbents are packed a s individual particles. In this month ‘s “Column Watch,” the guest columnists compare three of these novel sorbents with a conventional packed-particle column in terms of porosity, static and dynamic capacity. Monoliths show flow-rate-independent separation efficiency and dynamic capacity as well as higher porosity than conventional columns.