Characterization of Monolithic Convective Interaction Media® Columns
Monolithic chromatographic supports can efficiently be used for fast separation and purification of different types of molecules, both in the analytical and preparative scale. CIM Convective Interaction Media™ monolithic columns are macroporous polymeric supports that allow in-seconds separation of proteins and other biomolecules in gradient and isocratic modes.
In this work, the results showing the main characteristics of CIM™ columns are presented. The breakthrough curves at different flow rates were measured and it is shown that the dynamic binding capacity is practically unaffected by increased flow rates. The adsorption isotherm is almost rectangular exhibiting a highly favourable conditions for binding the tested components to the matrix. Furthermore, relatively high binding capacity is still maintained at elevated ionic strengths of the binding buffer. Finally, the HETP values of the components with different molecular masses are presented.