Characterization of various AAV subpopulation species, separated by multimodal column PrimaT

In each adeno associated virus (AAV) downstream process one of the key steps is enrichment of full capsids. This could be achieved by density gradient ultracentrifugation however a main drawback is its scalability. A more common approach is liquid chromatography using ion exchange chemistries, which based on particles’ charge differences, enables separation of full (F) capsids and product related impurities including non functioning AAV capsids (empty, partially filled, misfolded and wrongly packaged genome or other DNA containing subspecies).