Speakers:
Hena Divanovic, Application Scientist at Refeyn
Marko Narobe, Product Manager for High Throughput Solutions at Sartorius BIA Separations
Watch the webinar to learn how to:
- The use of CIM SO3 plates for high-throughput purification of AAV upstream samples, ensuring the necessary purity for analytics.
- Techniques to determine optimal production conditions for achieving the highest empty/full capsid ratio using mass photometry.
- Strategies to accelerate your production process with Ambr15.
Abstract:
This session will highlight new research focused on optimizing AAV6 upstream production – an essential step in improving the safety and affordability of gene therapies. Using a design of experiment (DoE) approach, the Sartorius BIA Separations team systematically identified the optimal conditions to achieve a high full capsid ratio. Further optimization was conducted with the Ambr15 system, while CIM SO3 monolithic plates enabled efficient high-throughput purification of upstream samples. Mass photometry was used to accurately assess capsid species ratios, supporting more precise and scalable production.
