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Mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) have promising therapeutic potential in diverse applications, such as tissue regeneration and immunomodulation. On the pathway to the clinic, there is a substantial need for development of scalable methods for EV production and isolation. Moreover, advancements in analytical tools for characterization of EVs are necessary to ensure a consistent product. Here, we showcase an integrative approach combining upstream, downstream and analytical processes for successful generation of MSC EVs.
