Transferring EV Purification Process from CIMmultus EV to QA HR Column

CIMmultus EV, an anion exchange column intended for the purification of extracellular vesicles and CIMmultus QA HR, a novel column from the CIM® QA HR line, designed for the highest batch-to-batch and scale-to-scale reproducibility, were used for isolation of extracellular vesicles following the same protocol. Both columns share the same chemistry, a quaternary amine ligand but slightly different ligand density. We demonstrate that the purification protocol developed for discontinued CIMmultus EV is straightway transferrable to the CIMmultus QA HR column. The comparability of both column types was demonstrated on elution profiles and recovery of extracellular vesicles.