The Power of Immunoaffinity Chromatography on CIMac Platform | Studying Glycosylation Profile of Fibrinogen in Human Plasma

The CIMac antibody immobilization platform enables an elegant immobilization of antibodies, which can be used as immunoaffinity adsorbents in specific diagnostic applications. Immobilization of antibodies to solid chromatographic supports is a very elegant solution for preparation of reusable immunoaffinity assays that can lower the price but also increase the accuracy and lower the limit of detection of biological assays for diagnostic purposes. Therefore, it is important that reusing a column for multiple sample analysis does not affect chromatographic/analysis performance.

In this application note, an example of reusability of the hydrazide-based CIMac column ( HDZ ) with immobilized monoclonal antifibrinogen for consecutive purification of fibrinogen from human plasma is shown.

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