Testing Different Activation Chemistries for IgG Immobilization Onto a Monolithic Support

Interactions between antibodies and their antigens are highly selective and therefore immensely popular for affinity chromatography. Consequently, numerous antibody immobilizations were performed on monolithic supports via different activation chemistries in the last decade. Despite the work already done there was no systematic study, where as many as possible activation chemistries were tested for the immobilization of a model monoclonal antibody with subsequent chromatographic characterization of the affinity support. In this work, various pre-activated CIM monolithic columns were used for the immobilization of a model monoclonal IgG.