Separation of Empty and Full AAV Capsids by Multimodal Metal Affinity Chromatography

Removal of empty capsids is a particular goal of AAV purification. Multimodal PrimaT ligand offers new options for removal of empty and also damaged capsids. Besides it also contributes to better clearance of contaminating DNA. PrimaT works for different AAV serotypes – for example AAV 2/8 and AAV 2/9.

AAV 2/8 or AAV 2/9 clarified harvest from Sf 9 cells was first processed by tangential flow filtration (TFF) coupled with Kryptonase treatment to reduce host cell DNA. Initial AAV capture step was performed on CIMmultus SO3 cation exchange column. After elution with sodium chloride gradient AAV fraction was cleared of DNA and protein contaminants.

Separation of empty and full AAV capsids was performed by multimodal metal affinity chromatography with CIMac and CIMmultus PrimaT.

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