Coupling trypsin directly onto a Convective interaction media (CIM) monolithic chromatographic support makes it possible to reuse the enzyme for multiple process cycles and to automate hydrolysis processes. Sartorius BIA Separations offers CIM trypsin immobilized enzyme reactors (trypsin IMERs) based on monoliths with volumes from 0.1 mL up to 80 mL) and with channel sizes of 2 µm or 6 µm to meet the needs of various applications. Common applications include sample preparation for mass spectrometry (MS)-based proteomics or industrial scale production of protein hydrolysates.
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