Rapid Desalting of E. Coli Cell Lysate for CGE Analysis Using Monolithic Spin Columns

Neutralized E. coli cell lysate is a complex intermediate sample in the production process of plasmid DNA (pDNA), which is a workhorse for use in modern vaccination as well as in gene and cell therapy. Lysate composition analysis is important in-process characterization which enables optimal planning of the pDNA upstream and downstream processing. pDNA isoform composition is often analyzed using capillary gel electrophoresis with laser induced fluorescence detection (CGE-LIF), a very sensitive and high-resolution method.

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