Purification and Analytics of pDNA Using Hydrophobic Monoliths in Sample Displacement Mode

CIM chromatographic monoliths enable high 1) productivity of pDNA downstream process (DSP) due to high dynamic binding capacity for pDNA in small elution volumes and short chromatographic runs; 2) high resolution power due to convective-based mass transfer.

Sample displacement mode utilizes different relative binding affinities of components in a sample mixture and separates pDNA isoforms under overloading conditions – where sc pDNA isoform acts as a displacer of oc or linear pDNA isoform.

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