Coupling oligo-deoxythymidilic acid (Oligo dT) probes to a monolith surface creates affinity chromatographic columns that selectively bind messenger RNA (mRNA) through hybridization with the mRNA’s poly-adenylic acid (poly A) tail.
This study presents the optimization of mRNA dynamic binding capacity (DBC) using CIMmic Oligo dT discs. These CIMmic discs, with a 100 μL bed volume, offer properties comparable to the preparative CIMmultus line and are ideal for screening samples, chromatographic conditions, and developing preparative purification methods for large biomolecules. The use of CIMmic discs significantly reduces sample consumption, making them invaluable in early development.
By utilizing CIMmic technology and down-scaling experiments, we were able to increase Oligo dT DBC5 from 1.9 mg/mL to 6.8 mg/mL by optimizing flow rate and buffer composition, using a limited amount of mRNA.
*The CIMmic discs discussed in this study are not yet available for commercial sale.
