Influence of Chromatographic Parameters on Empty | Full AAV Separation

Monoliths are commonly used for separation of empty and full AAV capsids on analytical and preparative scale. PrimaS approach for AAV separation employs an ascending pH gradient, which can be sensitive towards small changes in chromatographic parameters. In present work 200 µL testing units extracted from large CIMmultus PrimaS monolithic columns, were employed for the adjustment of critical chromatographic parameters.

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