This application note explores the impact of various parameters on performance of CIMmultus® PrimaT column to optimize initial recommended methods, tailoring them to user’s specific AAV sample for enhanced enrichment of full AAV. CIMmultus PrimaT is a multimodal column, allowing weak anion-exchange, hydrogen bonding and metal affinity coordination interactions. AAV serotype influences initial method selection. Screening different pH values critically impacts sample binding and alters elution profiles, affecting the separation of empty and full AAV capsids. The column’s scalability is demonstrated through experiments on AAV loading density and transitions from linear to step elution gradients. CIMmultus PrimaT allows binding of all AAV serotypes and provides advanced removal of product-related impurities and AAV subspecies, while maintaining high recovery rates.
Sartorius BIA Separations
