Estimation of Empty and Full AAV10 Particle Ratio Using the Multi-Angle Light Scattering Detector

Serotype 10 adeno-associated virus (AAVrh_10mCherry) was analysed on the PATfix system with the CIMac AAV full/empty analytical column to estimate the ratio of empty and full AAV particles based on the peak area of the chromatogram given with three different detectors. AAV included a protein capsid containing single stranded DNA. CIMac AAV column consisted of a strong anion exchanger with QA chemistry (quaternary amine).

Poster was prepared by Blaz Goricar and presented at ISBioTech 9th Spring Meeting where it was awarded the first prize. Congratulations!

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