Speakers:
Blaž Bakalar, Product Manager, PATfix® Platform, Sartorius BIA Separations
Michael Daume,Group leader, Downstream Process Development for Nucleic Acids, WACKER Biotech
Abstract:
Production of mRNA involves complex IVT mixtures, including target molecules, process- and product-related impurities, all with similar biophysical characteristics. Designing a correct hardware setup for reproducible analytical separation of these large biomolecules is a significant challenge. In this session, we explore innovative solutions for streamlining mRNA production.
Customer experience highlight:
Michael Daume, Group leader, Downstream Process Development for Nucleic Acids at WACKER Biotech, will discuss how the company develops novel manufacturing technologies and processes for both RNA and DNA manufacturing. These developments require sophisticated and easy-to-use analytical methods, such as HPLC-based techniques. In this presentation, Dr Daume will discuss how WACKER Biotech uses PATfix to gain valuable insights into the development of novel mRNA processes.
Feature demonstration:
In this presentation, we analyze in vitro transcription (IVT) reaction time points, providing insights into reaction kinetics by monitoring critical IVT components. The PATfix software simplifies sample visualization, enabling precise quality assessment and impurity identification. Its wide mRNA linearity range ensures accurate quantification through calibration curves. Additionally, the platform includes methods for polyadenylated mRNA quantification and robust quality control, separating samples by size to reveal abortive transcripts, target size molecules, and double-stranded RNA.
Key Takeaways:
- Understand the complexities of mRNA production and the challenges associated with separating large biomolecules.
- Learn how to accelerate your process development workflows with rapid feedback (5–15 minutes per sample) and user-friendly software for data processing, visualization, and sharing.
- Hear from scientists at WACKER Biotech, who have successfully implemented these solutions to optimize their mRNA production processes.
- Participate in an interactive Q&A session to get your questions answered and gain deeper insights into improving your mRNA production.
