Depletion of Human Serum Albumin in Embryo Culture Media for in Vitro Fertilization Using Immunoaffinity Chromatography

Bottom-up proteomic approach based on tandem mass spectrometry (MS/MS) is a method of choice for identification and quantitation of proteins in the complex biological samples. Followed by quantitative analysis of a protein sample, this approach enables the identification of putative biomarkers for early pathology diagnostics and differentiation. The main challenges confronting this analysis are suppression of low-abundance ions and the limited dynamic range of MS/MS. Affinity depletion of abundant proteins is an important stage in routine sample preparation prior to tandem mass spectrometry (MS/MS) analysis of biological samples. One such protein is Human Serum Albumin (HSA).

In this study, polyclonal αHSA antibodies were immobilized onto a chromatography support to use as immunoaffinity-based column (CIMac HDZ -αHSA) for HSA depletion from embryo culture media for in vitro fertilization (IVF).

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