A purification of synthetic oligonucleotides by using CIM monolith was evaluated. In this case study, the CIM anion exchange column had the capability to resolve oligonucleotides with small difference in comparative chain length.
A crude reaction mixture of synthetic oligonucleotide was loaded onto the CIM anion exchange column. Sample elution was achieved by salt concentration gradient. In comparison with conventional media, CIM monolith indicated higher resolution for major impurities.
Advantages of the characteristic properties of the CIM monolith were evaluated based on the high throughput purification of oligonucleotides under the identified gradient separation conditions. Over 99 % HPLC purity for the target oligo DNA was achieved by one-step purification from the crude reaction mixture.
