An Efficient End-to-End AAV8 Platform Process at 50 L Scale

This application note presents an end-to-end AAV production platform covering upstream and downstream processing exclusively using Sartorius technologies. In upstream processing, HEK293 cells were seeded from a 10 L rocking motion bioreactor into a 50 L stirred-tank reactor. The downstream process employed a range of purification techniques, such as clarification by filtration, TFF, and a serotype-independent capture step performed on a CIMmultus SO3 column, with a subsequent polishing chromatography step performed on a CIMmultus PrimaS column.