Speakers:
Blaž Bakalar, Product Manager, PATfix® Platform, Sartorius BIA Separations
Dan Peer, Chairman, NeoVac
Tristan Kovačič, Scientist, Process Analytics Development, Sartorius BIA Separations
Abstract:
Lipid nanoparticles (LNPs) are cell-specific delivery systems capable of encapsulating various payloads, including different nucleic acids. Their characterization proves to be a significant challenge due to their complex composition and structure.
Customer experience highlight:
Dan Peer from Tel Aviv University | NeoVac Ltd. and Tristan Kovacic from Sartorius BIA Separations share challenges and achievements in developing LNP formulations. NeoVac leveraged the PATfix LNP Platform to enhance the safety and reliability of their LNP-based therapeutics. The system has become an invaluable tool in their process development and optimization efforts, providing quick and comprehensive insights into every step of their LNP formulation process.
Feature demonstration:
The PATfix LNP Platform offers a unique characterization approach to LNP formulations, addressing the limitations of traditional assays that often require multiple time-consuming steps. Utilizing an innovative 2D chromatographic method with two different separation stages, the PATfix LNP Platform determines crucial LNP quality attributes in a single run without any sample preparation. Unlike other assays, it allows for simultaneous quantification of both encapsulated and naked nucleic acid cargo, including co-encapsulated species, and provides estimates of mRNA fragmentation and LNP size.
Key Takeaways:
- Understand the key parameters for characterizing LNPs.
- Discover liquid chromatography methods for encapsulation efficiency, lipid composition, load integrity, and size analysis.
- Hear from NeoVac how the PATfix LNP Switcher Platform has become crucial in enhancing the safety and reliability of LNP formulations by providing comprehensive insights into their development and optimization.
- Participate in an interactive Q&A session to enhance your LNP characterization processes.
