This application note demonstrates an advanced AAV E/F separation using CIMmultus QA HR. By leveraging CIMmultus QA HR columns built on the same QA ligand as CIMmultus QA but released through stringent QC based on empty AAV elution conductivity, the process achieves high reproducibility across all column scales.
QA HR line enables consistent full-capsid enrichment and purity, even when using step gradient. Available from 1 mL to 8 L, CIMmultus QA HR supports seamless lab-to-manufacturing scalability with reproducible performance at every scale. Coupled with an improved chromatographic method, this solution delivers reliable AAV purification and elevates downstream robustness to meet industrial manufacturing demands
