Speaker:
Klemen Božič, Scientist, Process Development mRNA | pDNA at Sartorius BIA Separations
Watch the webinar to learn how to:
- Tackle scale-up challenges in pDNA production, including effective control over lysis and chromatographic separation at large scale.
- Maximize recovery and consistency at gram-scale while adhering to regulatory standards.
- Implement continuous plasmid purification to maintain pDNA quality.
- Utilize at-line analytics to monitor and optimize purification in real time.
Abstract:
In this recorded webinar, discover effective strategies to tackle bottlenecks in plasmid (p)DNA downstream processing. Explore real-world process data to optimize each step—from lysis to sterile filtration—ensuring maximum yield, purity, and regulatory compliance.
Case studies showcase a fully integrated, end-to-end pDNA purification process, achieving over 98% integrity and 64% recovery of supercoiled pDNA, more than 95% sc pDNA in lysate with automated in-line lysis, over 90% recovery rates for AEX and HIC steps, and less than 4% loss in final UF/DF and sterile filtration.
Learn how real-time process monitoring with at-line analytics enhances performance and ensures quality.
