This application note thoroughly describes how small deviations in various chromatographic parameters can influence the separation of empty and full AAV (E/F AAV) capsids, using PrimaS® and PrimaS® HR columns.
Understanding how to ensure method reproducibility is key when dealing with delicate chromatographic separations. High control of parameters affecting the chromatographic method is essential to achieve consistent and reliable purification results. Even slight deviations in these parameters can result in changed outcomes of precise chromatography operations—examples being shifts in retention time, elution conductivity, and reduced peak resolution.
Although the following experiments focus on PrimaS HR, deviations in various chromatographic parameters have the same influence on both PrimaS and PrimaS HR columns.
