Sartorius In-Line Lysis System: The Easiest Alternative to Batch Alkaline Lysis

Start:
11
Jun
2024

Tue, Jun 11, 2024 10:00 AM EDT | 16:00 CEST

Speaker: Matevž Korenč, Product Manager, Sartorius BIA Separations

Abstract

Generally, methods applied for the isolation of plasmid DNA involve a cell lysis step with the addition of a strong base (alkaline lysis), neutralization, and precipitation to recover the target pDNA in solution from precipitated contaminating cell components (genomic DNA, proteins, and protein-nucleic acid complexes). Uncontrolled use of an alkaline solution will degrade target pDNA, which is also sensitive to mechanical stress. Furthermore, highly viscous solutions may cause local heterogeneities or require extensive mixing, both potentially degrading the target pDNA. This webinar will present a fully automated in-line lysis system with a closed single-use loop for process development up to a pilot scale in the GMP environment. With a state-of-the-art mixing control, it can be configured to automatically and continuously process up to 10–50 kg of resuspended cells per workday depending on the configuration.

Key Learning Objectives

  • Understand challenges associated with alkaline lysis at the pilot scale.
  • See the advantages presented by the Sartorius in-line lysis machine compared with batch lysis.
  • Learn about at-line in-process control strategies for challenging crude lysis samples.

About the Speaker

After receiving a PhD in Biochemistry, Matevz Korenc continued as a team leader in Knauf insulation, where he managed the analytical support and material development in the EMEA region. Matevz joined Sartorius BIA Separations in 2021 as a project manager and two years later became fully active in in-line lysis machine development and GMP implementation. He is also an acrobatic basketball performer, never found in his comfort zone.

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