2024
Speakers:
Justina Martinkiene, Scientist, Purification Sciences, AstraZeneca
Klemen Božič, Process Development Scientist, Sartorius BIA Separations
Date and Time: Tuesday, October 15, 2024 | 11 am EDT (NA) / 4pm BST (UK) / 5pm CEST (EU-Central)
Title: Implementation of an Improved Platform for Downstream Processing of Plasmid DNA
Abstract:
Plasmid DNA (pDNA) represents the backbone of most cell and gene therapy production workflows. As a key raw material, its quality can have an important effect on subsequent downstream processes. The increasing demand for highly purified pDNA at gram scale creates a need for controllable, robust, and scalable processes.
This webinar will provide insights into optimizing a process for capture of pDNA, and showcase data on a novel approach to removal of undesired pDNA isoforms. Attend for the opportunity to hear from purification scientists who will discuss the scientific challenges posed by the development process, resin and membrane screen study data, and their approach to working across different sizes of pDNA contruct while maintaining high yield and purity.
What You Will Learn
- How different types of chromatography media perform in capture of pDNA from clarified lysate
- The impact of size on the performance of chromatographic pDNA capture
- Use of selective binding of pDNA on hydrophobic chromatography to increase purity and decrease consumption of ammonium sulfate
- Implementing a platform for purification of any pDNA
