2026
In this webinar, attendees will explore how pH‑based denaturation and Oligo dT purification enable rapid dsRNA disruption, high mRNA recovery, and improved potency. Join Dr. Rok Sekirnik, head of process development of mRNA/pDNA at Sartorius BIA Separations and Evelin Nett, a PhD researcher specializing in RNA‑based technologies, as they share practical guidance on implementing this methodology in modern mRNA production to enhance mRNA integrity for personalized therapies and vaccines.
Key learning objectives:
- Learn how pH denaturation and Oligo dT purification enable selective dsRNA removal while maintaining high mRNA recovery and integrity.
- Understand how optimized dsRNA‑removal workflows can boost downstream performance, including 5-6x improved cell‑based potency.
- Gain insight how to apply this method using HPLC, FPLC, or centrifugation.
Who should attend?
- Scientists and developers working across upstream, downstream, analytical, and process development in mRNA and gene therapy workflows.
- R&D leaders, CDMOs, and specialists advancing therapeutic development and manufacturing strategies.
Speakers:
Rok Sekirnik, PhD, Head Process Development mRNA | pDNA, Sartorius BIA Separations
Dr. Rok Sekirnik graduated with a degree in chemistry from the University of Oxford, where he also completed his PhD. His team at Sartorius BIA Separations focuses on developing high-yielding, cost-effective purification approaches for pDNA and mRNA production.
Evelin Nett, PhD Student, TRON Translational Oncology, University Medical Center Mainz
Evelin Nett is a molecular biologist specializing in RNA-based technologies. She earned her Bachelor’s degree in Molecular Biology from Johannes Gutenberg University Mainz and her Master’s degree in Molecular Medicine from Johann Wolfgang Goethe University Frankfurt. She is currently pursuing her PhD at TRON Translational Oncology at the University Medical Center Mainz. Her research focuses on trans-amplifying RNA, with the goal of reducing intrinsic immunogenicity and improving performance for therapeutic and biomedical applications.
