4th Gene and Cell Therapies: CMC, CGT & Vector Manufacturing Summit

Listen to our presentation

Speaker: Aleš Štrancar, Managing Director, Sartorius BIA Separations
Time and Date: Thursday June 5, 2025 | 9:55 AM
Title: CMC, From pDNA and mRNA to LNP With Multiple Nucleic Acids Payloads

Presentation highlights:

• LNPs with multiple payloads are very sheer sensitive, therefore the TFF purification should be replaced with CIM monolith column chromatography deprived of shear forces.
• LNPs with multiple payloads are very heterogenous, chromatography approach is required to purify the LNP product.
• To enable the batch-to-batch product manufacturing consistency new analytical method to characterise the pDNA, mRNA and LNP should be used.

Location: München, Germany