PNGase F is an endoglycosidase commonly used for the selective release of N-glycans in glycan profiling. Alterations in the glycosylation pattern affects protein structure and function and has been correlated with several disease states. Assessment of the N-glycan profile - I.may shed light on the role of glycosylation in biological and pathological processes. - II.is required for the quality control of biotechnological drugs. Aim was to develop a PNGase F IMER that can be hyphenated with a LC-MS system for rapid and automated glycan analysis.