BioProcess International, October 2021
Abstract:Plasmid DNA (pDNA) has become a crucial component in ... grade pDNA has become a production bottleneck, leading to lengthy production delays.
There is a ...
downstream processing plasmid DNA (pDNA). Separation of pDNA using traditional particle-based anion ... -exchange supports is usually slow and has a low capacity for pDNA due to steric exclusion effects. Due ...
/s00232-013-9580-5
The use of plasmid DNA (pDNA) as a pharmaceutical tool has increased since it ... represents a safer vector for gene transfer compared to viral vectors. Different pDNA extraction ...
Černigoj
Electrophoresis. 2023; 1– 13
Increased need for plasmid DNA (pDNA) with sizes above 10 ... was to evaluate the mass balance and pDNA isoform composition at column outlet for plasmids of ...
(2010) 2039–2045Plasmid DNA (pDNA) used in vaccination and gene therapy has to be highly pure and ... purification of large molecules and nanoparticles seem to be a matrix of choice for pDNA purification. In ...
HPLC based analytical assay is a powerful technique that can be used to efficiently monitor plasmid DNA ... (pDNA) purity and quantity throughout the entire purification process. Anion exchange monolithic ...
ofpeptide and protein purification. Here, we report a use of SDC to separate plasmid DNA (pDNA ... concentration of AS.SDC was successfully implemented for the enrichment of sc pDNA for plasmid ...
shown to be superior to conventional particle-based column as plasmid DNA (pDNA) purification ... remaining endotoxin were subsequently removed to below detection limit with minimal loss of pDNA using ...
Chromatography A 1065 (2005) 93-106
The demand of high-purity plasmid DNA (pDNA) for gene-therapy and genetic ... vaccination is still increasing. For the large scale production of pharmaceutical grade plasmids ...
quantity of plasmid DNA (pDNA) throughout the purification process. However, the phenomenon of physical ... entrapment of open circular (OC) isoforms pDNA inside narrow channels of chromatographic support ...