Digital Week: mRNA Applications in Discovery & Development

Chromatography in mRNA Production: From Analytics to Purification

Speaker: Rok Sekirnik, Head of Process Development, mRNA I pDNA

Date & Time: December 8th, 9AM

Key message:

The session will describe case studies of on leveraging the power of at-line HPLC monitoring for process development to increase purity and yield of mRNA production. Approaches to optimize plasmid purification and linearization,  IVT reaction and mRNA purification will be described, including how to achieve >95% supercoiled plasmid purity, optimize linearization, and how to increase IVT reaction yield 3-5 mg/mL to 10-12 mg/mL. Use of single use bioreactors to produce multi-gram amounts of mRNA will be demonstrated. Purification with affinity, multimodal chromatography, reverse-phase chromatography and HIC will be shown, including multiparallel optimization approaches in 96-well format.

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