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Purification of mRNA with CIMmultus® PrimaS

The ligand in CIM® PrimaS contains a series of hydrogen donors and hydrogen acceptors, combined with a weak anion exchange character. Its hydrogen bonding ability makes its selectivity entirely distinct from traditional anion exchangers. PrimaS purifies large single-stranded mRNA (ssRNA) under aqueous conditions at ambient temperature. Contaminants are removed by means of a hight salt wash, while ssRNA is recovered from the column with a pH gradient. It can be used for purification or analytics of ssRNA, including RNA exceeding 10 kb (such as self-amplifying RNA). In a purification process, it is most often employed for capture of RNA in crude samples such as IVT mixtures.

CIMmultus® PrimaS combines anion exchange and hydrogen bonding properties. It binds molecules with predominantly negative charge and repels molecules with a predominantly positive charge. Samples are applied at neutral pH. A high salt wash is used to remove most contaminants, while ssRNA is eluted by increasing pH, which reduces the binding ability of the PrimaS column.