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Purification of mRNA with CIMmultus™ C4 HLD

CIMmultus C4 HLD is a hydrophobic interaction chromatography monolith for purification of nucleic acids. Sample is applied at high ionic strength in salts that precipitate RNA. The column is eluted with a decreasing salt gradient. The majority of DNA, dsRNA, and short transcripts elute earlier than intact ssRNA. The majority of proteins and aggregates bind very strongly and are eliminated by a cleaning step with NaOH. CIMmultus C4 HLD can be used to produce research grade ssRNA from in vitro transcription (IVT) mixtures but gives its best results as a polishing method.