Method development on CIM H-Bond monoliths
H-Bond ADC is the first of a new class of chromatography ligands from BIA separations that exploits hydrogen bonding as the dominant mode of biomolecule retention. The ligand consists of a terminal series of hydrogen donors grafted to a root series of hydrogen acceptors.
H-Bond ADC brings a unique new selectivity to all fractionation tasks but is especially distinctive in its ability to retain large biomolecules more strongly than small ones. This can be of great value for removing fragments, aggregates, and viruses from protein products, or for removing proteins and other small contaminants from large biologics like viruses and extra-cellular vesicles.