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Screening for Affinity Ligands on CIM® Minidisks

We have developed a screening procedure for peptide ligands for affinity chromatography on the same monolithic support. CIM® monolithic columns used conventionally for analytical and preparative separation of proteins and polynucleotides were minimized to fit into 96 well solid phase extraction plates. Peptide synthesis and screening were performed on the same format using a vacuum manifold for liquid throughput.