Design and characterisation of high throughput 8 mL radial flow monolithic chromatographic column
There are two different designs of chromatographic columns concerning the flow profile. Most of today's HPLC columns belong to the group of so-called axial mode operating columns, while the radial ones with a radial flow pattern are more rare. Which type performs better depends on the particular case but it seems that the radial operating columns are attracting interest since they exhibit some beneficial features. One of the main problems of radial operating chromatographic columns is the changing of a mobile phase linear velocity over the chromatographic bed. Because of that, matrix efficiency for porous particulate supports varies by its position within the bed, and overall performance is more difficult to predict.
This problem is not present when the monolithic supports are used, since it was demonstrated that their chromatographic properties are flow unaffected even at the extreme linear velocities. This was confirmed also for the radial operating mode.
The monolith and radial flow housing were designed for extremely high flow rates, up to 70 CV/min, which is the range of the flow rates applied on membranes. This was achieved by proper monolith dimensions with the height of 55 mm, inner diameter of 6.0 mm and thickness of only 4.5 mm.